2024 Jeg-3 cell line

Jeg-3 cell line

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August undefined, 2024

Web10 nov 2006 · The JEG-3 cell line are human choriocarcinoma cells derived from malignant placental tissues. As in placenta, JEG-3 cells endogenously express aromatase activity at detectable levels (Bahn et al., 1981) and the cyp19 gene expression is under the control of a distal promoter I.1 located adjacently upstream of exon I.1 in the gene (Means et al ... http://hs.read.cnki.net.dr2am.wust.edu.cn/web/Journal/Article/WJDE202411006.html?__dp=https

The choriocarcinoma cell line JEG-3 upregulates regulatory T cell ...

Web13 nov 2013 · Line Spacing: Column ... MCF7 and HepG2 cells were transiently transfected with 3 μg HIS-FLAG-tagged WT Nr5a1 or Nr5a1 in which lysine 194 (K194R), glutamic acid 199 (E199A), or both lysine 194 and glutamic acid 199 (K194RE199A) were mutated to arginine or alanine. Web1 mag 2002 · Due to the metabolic property demonstrated in this study, the JEG-3 cell line belongs to a little group of transformed and established cell lines experimentally shown … luxury train travel in scotland

Matrix metalloproteinase-2 (MMP-2) generates soluble HLA-G1 by cell …

WebThe JEG-3 line cells were treated by 4 µmol CFSE, then 3 × 10 4 JEG-3 line cells in 50 µL of DMEM medium were inserted in 96-well plates with 10% FCS. NK-92 line cells in 100 … WebCell line: JEG-3 : DSMZ no.: ACC 463: Species validation: human (Homo sapiens)Cell type: choriocarcinoma : Origin: established from a human gestational choriocarcinoma … WebMIR503HG was expressed predominantly in the nucleus of JEG-3 choriocarcinoma cells. ... the three-exon LINC00629 new isoforms … luxury train travel deals

Cellosaurus cell line JEG-3 (CVCL_0363)

Web1 dic 2024 · The aim was to evaluate changes in the NK cell phenotype and function in the presence of IL-15, IL-18 and JEG-3, a trophoblast cell line. When cocultured with JEG-3 … WebWe measured the basal levels of phosphorylated ATR kinase in SW1353, CS-1, 5-8F, and JEG-3 cells. The results showed that the chondrosarcoma CS-1 cell line and SW1353 cells expressed very low levels of phosphorylated ATR at baseline. As experimental controls, 5-8F and JEG-3 expressed moderate levels of phosphorylated ATR . kings arms oldham contact numberJEG-3 is a hypertriploid, clonally-derived, human cell line with epithelial morphology that was isolated from the Woods strain of the Erwin-Turner tumor. This cell line is tumorigenic, capable of forming malignant tumors consistent with choriocarcionoma. Genes expressed: human chorionic gonadotropin (hCG); human chorionic somatomammotropin ... luxury train travel usa east coast

"Web12 nov 2024 · The JEG-3 cell line was transfected with pSpCas9 vector cloned with each sgRNA and analysed as follows. First, HLA-G expression was measured by WB and FC on JEG-3 edited cells. " - Jeg-3 cell line

Jeg-3 cell line

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WebFurthermore, retinoids are secreted from JEG-3, as shown by the activation of retinoic acid-responsive beta lacZ reporter cells grown in conditioned media. These results suggest … Web1 gen 2024 · JEG-3 is another independent human choriocarcinoma cell line reported first in 1971 [9]. It is mostly considered a model for invasive trophoblast cells [ 6 ]. JEG-3 …

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Web29 gen 2024 · The JEG-3 choriocarcinoma cell line serves as a model to study EVT cell behavior in the presence of . MPO as primary trophoblasts lack su fficient proliferation in vitro (22). Web1 dic 2016 · Results: Our results showed that HTR-8/SVneo cell line contains two populations of cells suggesting the presence of trophoblast and stromal/mesenchymal cells. While all cells in BeWo, JEG-3 and ...

WebOne of 6 clones derived from cells implanted into a hamster cheek pouch and then propagated on irradiated feeder layers of human fibroblasts. ... JEG-3 Product Name: … WebIn this study, we found that the human HLA-G-positive choriocarcinoma cell line JEG-3 upregulates CD4 + CD25 hi CD127 lo T cells, increases the expression of HLA-G + CD4 + and CD8 + T cells, and decreases the expression of ILT2 + on CD4 + T cells in resting PBMCs after six days of co-culture.

Web31 mar 2024 · The choriocarcinoma-derived JEG-3 cell-line (ATCC HTB-36, Manassas, VA, USA), was grown as recommended by ATCC: ... JEG-3 cells in 2.5% FBS released similar levels of hCG, hPL, and estradiol to JEG-3 cells in 10% FBS, and thus maintain the endocrine function of human placenta. WebJEG, JEG-3 Human choriocarcinoma One of 6 clones derived from cells implanted into a hamster cheek pouch and then propagated on irradiated feeder layers of human fibroblasts. Cell line releases chorionic gonadotrophin and somatomammotrophin and progesterone. It is also able to transform steroid precursors to oestrone and oestradiol. JEG3 has the …

Web6 ott 2005 · Like the native trophoblast HLA-G, the JEG-3 cell HLA-G heavy chain is recognized by both of the monoclonal antibodies (mAb) (2C/C8 and 3C/G4) used in our enzyme-linked immunosorbent assay (ELISA) (see below). Using a cell line had the advantage of avoiding contamination by other cell types that may also have …

Web15 gen 2024 · JEG-3 cells synthesize placenta-specific hormones, such as human chorionic gonadotrophin [1] and, in contrast to most other “trophoblastic” cell lines, … luxury train tours england kings arms ocean city mdWeb9 mag 2024 · The miR-371-3 cluster (including miR-371, -372, and -373) is expressed in primary trophoblast cells, JEG-3 cells, and their hybrids, but not in immortalized HTR-8/SVneo Mice have homologous ... regulation of Wnt/ β-catenin signaling pathway by miR-424-5p and affects invasion and migration of the trophoblastic cell line HTR-8/SVneo kings arms ocean city marylandWebThe cells preserved in our CM-ACF freeze medium can be used for cell counting, viability and cryopreservation, cell culture, mammalian cell culture, gene expression analysis … kings arms old broad streetWebThe Jeg-3 cell line was used as a positive control. Results: Membrane-bound HLA-G expression levels decreased significantly in both cell lines after transfection with both … kings arms offham menuWebHuman choriocarcinoma trophoblastic cells (JEG-3) were used as positive control for the constitutive expression of HLA-G [53]. ... Firstly, we conﬁrmed that 7221.221 B-LCL cell line did not express any HLA antigen (Fig. 1a, b), while 721.221-HLA-G1-transfected cell line (221-G1) constitutively luxury train travel in the united statesWeb13 apr 2024 · To verify the function of miR-526b-5p on the trophoblast cells and explore the intrinsic molecular mechanism, transcriptome sequencing was performed on the HTR8 … kings arms otterton facebook