Web但是，与BrdU一样，EdU也有毒性，EdU的掺入会对裂殖的DNA产生损伤，影响细胞的连续繁殖[41]。 同样类似的毒害效应也发生在EdU标记动物细胞基因组DNA时，表明EdU只能用于短时期和单细胞周期的实验标记，而BrdU则适用于连续标记多个细胞周期[5]。 WebBromodeoxyuridine ( 5-bromo-2'-deoxyuridine, BrdU, BUdR, BrdUrd, broxuridine) is a synthetic nucleoside analogue with a chemical structure similar to thymidine. BrdU is commonly used to study cell proliferation in living tissues [1] and has been studied as a radiosensitizer [2] and diagnostic tool in people with cancer. [3]
DNA损伤与修复 - 简书
WebThe Invitrogen Click-iT EdU cell proliferation assays combine EdU labeling with powerful click chemistry to provide a superior alternative to traditional BrdU staining methods for detecting and quantitating newly synthesized DNA. Fast —Detection in as little as 80 min, without an antibody. Mild conditions —No denaturation required. WebBrdU labeling: in vitro labeling of cells with BrdU. Prepare a 10 mM stock solution of BrdU (ab142567) by dissolving 3 mg of BrdU in 1 mL water. Dilute the 10 mM BrdU stock … counties in westchester ny
EdU细胞增殖检测 锐博生物 - RiboBio
WebBromodeoxyuridine (BrdU) incorporation assays have long been used to detect DNA synthesis in vivo and in vitro. The key principle of this method is that BrdU incorporated as a thymidine analog into nuclear DNA represents a label that can be tracked using antibody probes. In this chapter, we describe BrdU incorporation into limbal stem cells. Web直接测定DNA合成是细胞增殖检测的最准确方法之一，是测定物质毒性、评估药物安全评价、细胞健康的基本方法，其中以前常用的方式是利用胸腺嘧啶核苷酸类似物——BrdU进行检测。因为在细胞周期的S期，和细胞一起孵育的BrdU能渗入DNA分子中，再结合BrdU抗体与渗入DNA的BrdU特异性结合，就能够检测 ... WebDNA损伤过程中可形成某些特定的损伤产物，可通过检测损伤产物（磷酸化H2AX、8-OHdG等）来评估DNA损伤。. 也可标记损伤DNA，如末端脱氧核苷酸转移酶介导的脱氧 … brentwood ccg